H2N2V1, Main, Exploration, bibRecord, 001030

The influence of the multi-basic cleavage site of the H5 hemagglutinin on the attenuation, immunogenicity and efficacy of a live attenuated influenza A H5N1 cold-adapted vaccine virus

Identifieur interne : 001030 ( Main/Exploration ); précédent : 001029; suivant : 001031

The influence of the multi-basic cleavage site of the H5 hemagglutinin on the attenuation, immunogenicity and efficacy of a live attenuated influenza A H5N1 cold-adapted vaccine virus

Auteurs : Amorsolo L. Suguitan ; Michael P. Marino ; Purvi D. Desai ; Li-Mei Chen ; Yumiko Matsuoka ; Ruben O. Donis ; Hong Jin ; David E. Swayne ; George Kemble ; Kanta Subbarao

Source :

Virology [ 0042-6822 ] ; 2009.

RBID : PMC:2787913

Descripteurs français

English descriptors

KwdEn :
- Adaptation, Physiological, Amino Acid Sequence, Animals, Base Sequence, Chickens, Cold Temperature, DNA, Viral, Drug Administration Schedule, Female, Hemagglutinin Glycoproteins, Influenza Virus (chemistry), Influenza A Virus, H5N1 Subtype (physiology), Influenza Vaccines (immunology), Mice, Mice, Inbred BALB C, Orthomyxoviridae Infections (prevention & control), Recombinant Proteins, Vaccines, Attenuated (immunology), Viral Proteins.
MESH :
- chemical , chemistry : Hemagglutinin Glycoproteins, Influenza Virus.
- chemical , immunology : Influenza Vaccines, Vaccines, Attenuated.
- chemical : DNA, Viral, Recombinant Proteins, Viral Proteins.
- physiology : Influenza A Virus, H5N1 Subtype.
- prevention & control : Orthomyxoviridae Infections.
- Adaptation, Physiological, Amino Acid Sequence, Animals, Base Sequence, Chickens, Cold Temperature, Drug Administration Schedule, Female, Mice, Mice, Inbred BALB C.

Abstract

A recombinant live attenuated influenza virus ΔH5N1 vaccine with a modified hemagglutinin (HA) and intact neuraminidase genes from A/Vietnam/1203/04 (H5N1) and six remaining genome segments from A/Ann Arbor/6/60 (H2N2) cold-adapted (AA ca) virus was previously shown to be attenuated in chickens, mice and ferrets. Evaluation of the recombinant H5N1 viruses in mice indicated that three independent factors contributed to the attenuation of the ΔH5N1 vaccine: the attenuating mutations specified by the AA ca loci had the greatest influence, followed by the deletion of the H5 HA multi-basic cleavage site (MBS), and the constellation effects of the AA genes acting in concert with the H5N1 glycoproteins. Restoring the MBS in the H5 HA of the vaccine virus improved its immunogenicity and efficacy, likely as a consequence of increased virus replication, indicating that removal of the MBS had a deleterious effect on the immunogenicity and efficacy of the ΔH5N1 vaccine in mice.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2787913

DOI: 10.1016/j.virol.2009.09.017
PubMed: 19833372
PubMed Central: 2787913

Affiliations:

Le document en format XML

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<author><name sortKey="Desai, Purvi D" sort="Desai, Purvi D" uniqKey="Desai P" first="Purvi D." last="Desai">Purvi D. Desai</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The influence of the multi-basic cleavage site of the H5 hemagglutinin on the attenuation, immunogenicity and efficacy of a live attenuated influenza A H5N1 cold-adapted vaccine virus</title>
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<wicri:noCountry code="subfield">USA 30605</wicri:noCountry>
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<term>Animals</term>
<term>Base Sequence</term>
<term>Chickens</term>
<term>Cold Temperature</term>
<term>DNA, Viral</term>
<term>Drug Administration Schedule</term>
<term>Female</term>
<term>Hemagglutinin Glycoproteins, Influenza Virus (chemistry)</term>
<term>Influenza A Virus, H5N1 Subtype (physiology)</term>
<term>Influenza Vaccines (immunology)</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Orthomyxoviridae Infections (prevention & control)</term>
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<term>Vaccines, Attenuated (immunology)</term>
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<term>Animaux</term>
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<term>Calendrier d'administration des médicaments</term>
<term>Femelle</term>
<term>Glycoprotéine hémagglutinine du virus influenza ()</term>
<term>Infections à Orthomyxoviridae ()</term>
<term>Poulets</term>
<term>Protéines recombinantes</term>
<term>Protéines virales</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Sous-type H5N1 du virus de la grippe A (physiologie)</term>
<term>Séquence d'acides aminés</term>
<term>Séquence nucléotidique</term>
<term>Vaccins antigrippaux (immunologie)</term>
<term>Vaccins atténués (immunologie)</term>
</keywords>
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</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Influenza Vaccines</term>
<term>Vaccines, Attenuated</term>
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<term>Recombinant Proteins</term>
<term>Viral Proteins</term>
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<term>Vaccins atténués</term>
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<term>Base Sequence</term>
<term>Chickens</term>
<term>Cold Temperature</term>
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<front><div type="abstract" xml:lang="en"><p id="P4">A recombinant live attenuated influenza virus ΔH5N1 vaccine with a modified hemagglutinin (HA) and intact neuraminidase genes from A/Vietnam/1203/04 (H5N1) and six remaining genome segments from A/Ann Arbor/6/60 (H2N2) cold-adapted (AA <italic>ca</italic>
) virus was previously shown to be attenuated in chickens, mice and ferrets. Evaluation of the recombinant H5N1 viruses in mice indicated that three independent factors contributed to the attenuation of the ΔH5N1 vaccine: the attenuating mutations specified by the AA <italic>ca</italic>
 loci had the greatest influence, followed by the deletion of the H5 HA multi-basic cleavage site (MBS), and the constellation effects of the AA genes acting in concert with the H5N1 glycoproteins. Restoring the MBS in the H5 HA of the vaccine virus improved its immunogenicity and efficacy, likely as a consequence of increased virus replication, indicating that removal of the MBS had a deleterious effect on the immunogenicity and efficacy of the ΔH5N1 vaccine in mice.</p>
</div>
</front>
</TEI>
<affiliations><list></list>
<tree><noCountry><name sortKey="Chen, Li Mei" sort="Chen, Li Mei" uniqKey="Chen L" first="Li-Mei" last="Chen">Li-Mei Chen</name>
<name sortKey="Desai, Purvi D" sort="Desai, Purvi D" uniqKey="Desai P" first="Purvi D." last="Desai">Purvi D. Desai</name>
<name sortKey="Donis, Ruben O" sort="Donis, Ruben O" uniqKey="Donis R" first="Ruben O." last="Donis">Ruben O. Donis</name>
<name sortKey="Jin, Hong" sort="Jin, Hong" uniqKey="Jin H" first="Hong" last="Jin">Hong Jin</name>
<name sortKey="Kemble, George" sort="Kemble, George" uniqKey="Kemble G" first="George" last="Kemble">George Kemble</name>
<name sortKey="Marino, Michael P" sort="Marino, Michael P" uniqKey="Marino M" first="Michael P." last="Marino">Michael P. Marino</name>
<name sortKey="Matsuoka, Yumiko" sort="Matsuoka, Yumiko" uniqKey="Matsuoka Y" first="Yumiko" last="Matsuoka">Yumiko Matsuoka</name>
<name sortKey="Subbarao, Kanta" sort="Subbarao, Kanta" uniqKey="Subbarao K" first="Kanta" last="Subbarao">Kanta Subbarao</name>
<name sortKey="Suguitan, Amorsolo L" sort="Suguitan, Amorsolo L" uniqKey="Suguitan A" first="Amorsolo L." last="Suguitan">Amorsolo L. Suguitan</name>
<name sortKey="Swayne, David E" sort="Swayne, David E" uniqKey="Swayne D" first="David E." last="Swayne">David E. Swayne</name>
</noCountry>
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The influence of the multi-basic cleavage site of the H5 hemagglutinin on the attenuation, immunogenicity and efficacy of a live attenuated influenza A H5N1 cold-adapted vaccine virus

The influence of the multi-basic cleavage site of the H5 hemagglutinin on the attenuation, immunogenicity and efficacy of a live attenuated influenza A H5N1 cold-adapted vaccine virus

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Abstract

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